绵羊AA-NAT基因mRNA表达与常年发情相关性研究

doi:10.11843/j.issn.0366-6964.2015.04.005

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (4): 542-548.doi: 10.11843/j.issn.0366-6964.2015.04.005

绵羊AA-NAT基因mRNA表达与常年发情相关性研究

卢璐璐¹，孙晓笛²，储明星^1*，王永娟²，黄冬维¹，王金玉²，狄冉¹，潘章源¹，刘秋月¹

（1.中国农业科学院北京畜牧兽医研究所，农业部畜禽遗传资源与种质创新重点实验室，北京 100193；2.扬州大学动物科学与技术学院，扬州 225009）

收稿日期:2014-05-05 出版日期:2015-04-23 发布日期:2015-04-23
通讯作者: 储明星，研究员，E-mail：mxchu@263.net
作者简介:卢璐璐(1990-)，女，安徽宿州人，硕士生，主要从事动物分子遗传学研究，E-mail：lulululu2012@sina.cn
基金资助:
国家自然科学基金项目(31101687)；中国农业科学院科技创新工程（ASTIP-IAS13）；国家肉羊产业技术体系专项(CARS-39)；中央级公益性科研院所基本科研业务费专项(2011cj-7；2013ywf-zd-1)

Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep

LU Lu-lu¹，SUN Xiao-di²，CHU Ming-xing^1* ，WANG Yong-juan²，HUANG Dong-wei¹，WANG Jin-yu²，DI Ran¹，PAN Zhang-yuan¹，LIU Qiu-yue¹

(1.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture，Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China；2.College of Animal Science and Technology，Yangzhou University，Yangzhou 225009，China)

Received:2014-05-05 Online:2015-04-23 Published:2015-04-23

摘要/Abstract

摘要：

为了探讨AA-NAT基因表达与绵羊常年发情之间的关联性，本研究以常年发情小尾寒羊和季节性发情滩羊各9只为研究对象，利用real-time PCR技术检测AA-NAT基因在这2种绵羊不同发情阶段下丘脑、垂体、松果体、卵巢、子宫体和肾上腺6种组织中的mRNA表达情况。结果显示，AA-NAT基因在不同情期的两个绵羊品种的6个组织中表达有明显差异，在发情期绵羊的卵巢、子宫体、松果体中高水平表达。春秋季发情期小尾寒羊与秋季发情期滩羊相比，其卵巢和子宫体中AA-NAT基因表达量极显著偏低（P<0.01），而松果体中表达量极显著偏高（P<0.01）。本研究结果初步表明，卵巢、子宫体和松果体中AA-NAT基因表达上调可能与小尾寒羊常年发情相关。

Abstract:

In order to study the association of AA-NAT gene and year-round estrus in sheep，year-round estrous Small Tail Han ewes and seasonal estrous Tan ewes were selected as tested subjects.Real-time PCR method was used to detect AA-NAT mRNA expression in hypothalamus，pituitary gland，pineal gland，ovary，uterus body and adrenal gland at different estrus stages.The results showed that the expression of AA-NAT gene in all 6 tissues at different estrous stages of the 2 breeds was significantly different.The expression of AA-NAT gene was at high level in ovary，uterus body and pineal gland in sheep at estrus.In ovary and uterus body， AA-NAT gene expression was significantly lower (P<0.01) in Small Tail Han sheep at spring and fall estrus stage than that in Tan sheep at fall estrus stage，but in pineal gland it was significantly higher (P<0.01).The results preminarily indicated that the up-regulated expression of AA-NAT gene in ovary，uterus body and pineal gland was associated with year-round estrus in Small Tail Han sheep.

中图分类号:

S826
S814.8

卢璐璐，孙晓笛，储明星，王永娟，黄冬维，王金玉，狄冉，潘章源，刘秋月. 绵羊AA-NAT基因mRNA表达与常年发情相关性研究[J]. 畜牧兽医学报, 2015, 46(4): 542-548.

LU Lu-lu，SUN Xiao-di，CHU Ming-xing，WANG Yong-juan，HUANG Dong-wei，WANG Jin-yu，DI Ran，PAN Zhang-yuan，LIU Qiu-yue. Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 542-548.

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绵羊AA-NAT基因mRNA表达与常年发情相关性研究

Association between AA-NAT mRNA Expression and Year-round Estrus in Sheep

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